T4 ligase

關於「T4 ligase」標籤，搜尋引擎有相關的訊息討論：

T4 DNA Ligase | NEBT4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.Ligation Protocol with T4 DNA Ligase (M0202) | NEBProtocols.io also provides an interactive version of this protocol where you can discover and share optimizations with the research community.T4 DNA ligase structure reveals a prototypical ATP-dependent ...2018年8月29日 · Bacteriophage T4 DNA ligase is the first ATP-dependent ligase ... Our crystal structure of T4 DNA ligase bound to DNA shows a ... Pascal J.M., Tsodikov O.V., Hura G.L., Song W., Cotner E.A., Classen S., Tomkinson A.E., Tainer J.A., Ellenberger T.. ... Maroney P.A., Chamnongpol S., Souret F., Nilsen T.W..Functional characterization of the T4 DNA ligase: a new insight into ...Here we report a functional characterization of the T4 DNA ligase. ... Carter K, Caldecott K, Pappin DJ, Yu GL, Wang RP, Shell BK, Nash RA, Schär P, et al.The Inhibitory Effect of Non-Substrate and Substrate DNA on the ...2016年3月8日 · It is demonstrated that not only does non-nicked dsDNA inhibit steady state ligation rates for T4 DNA ligase, but that the level of inhibition was ...T4 DNA Ligase - Takara BioBoth blunt and cohesive end DNA ligation, as well as single-stranded nick repair of DNA, RNA and DNA/RNA, are possible using the T4 DNA ligase.The phage T4 DNA ligase in vivo improves the survival-coupled ...2019年6月13日 · Contributions. JW and FL contributed equally to this work. FL performed experiments and analyzed data. JW, TS, YC and QG performed some ...Two-tiered enforcement of high-fidelity DNA ligation | Nature ...2019年11月28日 · DNA ligases catalyze the joining of DNA strands to complete DNA ... DNA ligase 1 (LIG1) discriminates against DNA junctions harboring ... G. L. APE1-dependent repair of DNA single-strand breaks containing 3'-end 8-oxoguanine. ... 111 TW Alexander Drive, Research Triangle Park, NC, 27709, USA.A ligation-triggered and protein-assisted fluorescence anisotropy ...Subsequently, 2 U DNA ligase (T4) and different concentration of ATP were added to the buffer to initiate the ligation reaction at 25 °C for 0.5 h. Then the buffer ...Drug Toxicity in Embryonic Development I: Advances in ...Johnson AL, Barker DG, Johnston LH (1986) Induction of yeast DNA ligase genes in ... Teratology 47: 229–242 Kimmel CA, Claggett TW, Kimmel GL, Tudor N, ...