T4 Ligase Promega

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T4 DNA Ligase - Promega CorporationJoins Two DNA Strands with Cohesive or Blunt Ends. Use with 5´, 3´ or blunt-ended dsDNA (e.g., inserts and vectors); Qualified for blue/white cloning, ... | [PDF] T4 DNA Ligase Protocol - Promega Corporationall Promega products to prevent direct human contact. ... Enzyme Storage Buffer: T4 DNA Ligase is supplied in 10mM Tris-HCl (pH 7.4), 50mM KCl, 1mM DTT, ... twA Versatile Zero Background T-Vector System for Gene Cloning and ...2009年4月21日 · Ligations using Promega T4 DNA ligase, the same product used by the ... Wang GL (2006. b) A highly efficient transient protoplast system for ...[PDF] T and pGEM(R)-T Easy Vector Systems Technical Manual #TM042Alternatively, if the maximum number of transformants is required, incubate the reactions overnight at 4°C. Notes: 1. Use only Promega T4 DNA Ligase supplied ...Promega T4 DNA Ligase - Modifying Enzymes - Fisher ScientificFor ligation of DNA ends. Shop Promega T4 DNA Ligase at Fishersci.com. | Cloning with pGEM®-T Vectors: Ligation - Promega Connections2020年1月8日 · The next step after adding an A-tail to a blunt-ended DNA fragment for T-Vector Cloning is ligation preparation. | T4 DNA Ligase | NEBT4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.DNA Ligation Protocol - Sigma-AldrichThis kit contains T4 DNA ligase, the enzyme of choice for virtually all cloning purposes because of its ability to ligate both cohesive and blunt-ended strands ...T4 DNA Ligase from Promega | Biocompare.comT4 DNA Ligase catalyzes the joining of two strands of DNA between the 5'-phosphate and the 3'-hydroxyl groups of adjacent nucleotides in either a ... twDNA Repair, Part ATerminal deoxynucleotidyl transferase is available from Promega (Madison, WI). ... 1997), uracil DNA glycosylase (Slupphaug et al., 1995), and DNA ligase I ...