ddNTPs

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A system for rapid DNA sequencing with fluorescent chain ... - ScienceJM Prober,; GL Trainor,; RJ Dam,; FW Hobbs,; CW Robertson,; RJ Zagursky, ... based on the use of a novel set of four chain-terminating dideoxynucleotides, ... tw | twA system for rapid DNA sequencing with fluorescent chain ... - Science... for rapid DNA sequencing with fluorescent chain-terminating dideoxynucleotides. JM Prober,; GL Trainor,; RJ Dam,; FW Hobbs,; CW Robertson,; RJ Zagursky, ... tw | twImproving dideoxynucleotide-triphosphate utilisation by the hyper ...Bacteriophage T7 DNA polymerase incorporates ddNTPs much more efficiently than the ... Prober J.M., Trainer,G.L., Dam,R.J., Hobbs,F.W., Robertson,C.W., ... tw | twRNA sequencing using fluorescent-labeled dideoxynucleotides and ...Although dideoxy terminated sequencing of RNA, using reverse transcriptase and oligodeoxynucleotide primers, is now a well established method, the accuracy is ... tw | twRole of Q190 of MuLV RT in ddNTP resistance and fidelity of DNA ...To obtain some insight into the structural basis for the differential utilization of dNTP and ddNTP by the mutant enzymes, we modeled the binary and the ternary ...New dye-labeled terminators for improved DNA sequencing patternsDye-labeled primer sequencing has benefited from the development of DNA polymerases which do not discriminate between deoxy- and dideoxynucleotides ( 7 ...Thermococcus sp. 9°N DNA polymerase exhibits 3'-esterase activity ...2019年6月20日 · However, the linker was absent from 3′-TL and 3′-GL incorporation ... dNTPs, though a 2x concentration is required for the former (Fig.A method for DNA sequencing using RNA polymerase | PNASDye-primer chemistry is useful for long-read sequencing due to the uniform incorporation of four types of ddNTP, resulting in an even peak height for each ... tw | twImproved DNA Sequencing Accuracy and Detection of ...The most versatile means of automated DNA sequencing uses dye terminators, which are dideoxynucleotide triphosphates (ddNTPs) labeled with different ...DNA sequencing using biotinylated dideoxynucleotides and mass ...The structure of the biotinylated dideoxynucleotides used to generate Sanger ... Ju,J., Glazer,A.N. and Mathies,R.A. (1996) Cassette labeling for facile.


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