Quantification of mRNA using real-time RT-PCR - Nature
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Real-time PCR (qPCR) uses fluorescent reporter dyes to combine the amplification and detection steps of the PCR reaction in a single tube format ... Skiptomaincontent Thankyouforvisitingnature.com.YouareusingabrowserversionwithlimitedsupportforCSS.Toobtain thebest
延伸文章資訊
- 1Reverse transcription polymerase chain reaction - Wikipedia
Protocol
- 2Universal SYBR Green qPCR Protocol - Sigma-Aldrich
Real-time or Quantitative PCR and RT-PCR use the linearity of DNA amplification to determine abso...
- 3QIAGEN OneStep RT-PCR Handbook
The protocol has been optimized for 1 pg – 2 µg of total RNA. Optimal reaction conditions, such a...
- 4Basic Principles of RT-qPCR | Thermo Fisher Scientific - TW
RNA as the Starting Material ... Quantitative reverse transcription PCR (RT-qPCR) is used when th...
- 5RT-PCR: Two-Step Protocol Two-step Protocol
Alternatively, RT-PCR can be done in two steps, first with the reverse transcription and then the...
