Steps of PCR - Extension - LabCE.com, Laboratory ...
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Overlap extension PCR · Annealing parts of primer: Solid arrow in scheme; Should bind to template...
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Extension is achieved by using the loosened nucleotides of each base to grow the complementary DN...
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Extension Time · Extensions are normally performed at 68°C · As a general rule, use extension tim...
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Final Extension for 5 minutes at 72°C: A final extension to fill-in any protruding ends of the ne...
