Unmodified dideoxy-Nucleoside triphosphates (ddNTPs ...
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Youarehere: | | | | Unmodifieddideoxy-Nucleosidetriphosphates(ddNTPs) Dideoxynucleotidetriphosphates(ddNTPs)lackthe3'-OHgroupofdNTPsthatisessentialforpolymerase-mediatedstrandelongationinaPCR.Therefore,ddNTPsareusedincombinationwithamodifiedTaqpolymerase(e.g.JBSSequencingp
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It relies on the extension of a primer, designed to bind one nucleotide upstream of the polymorph...
