Mind Your P's And Q's: A Short Primer On Proofreading ...
文章推薦指數: 80 %
Forapplicationssuchassite-directedmutagenesis,itisoftenrecommendedthatyouuseaproofreadingpolymerase(alsoknownashigh-fidelitypolymerases)tominimizetheriskofintroducingunintendedpointmutations. Butwhatisaproofreadingpolymerase? Whatmakesthemdifferentfromotherpolymerases? Andwhens
延伸文章資訊
- 1Taq DNA Polymerases - 圖爾思公司-最強最專業技術服務與 ...
The extension rate of TOOLS Taq DNA Polymerase is 1-2 kb/min in PCR. ... activity and a 5'→3' exo...
- 2Platinum™ <i>Taq</i> DNA Polymerase High Fidelity
High fidelity is provided by a mixture of Platinum® Taq DNA Polymerase and the proofreading (3´→5...
- 3DNA Polymerase Proofreading | NEB
It allows the enzyme to check each nucleotide during DNA synthesis and excise mismatched nucleoti...
- 4Taq polymerase - Wikipedia
One of Taq's drawbacks is its lack of 3' to 5' exonuclease proofreading activity resulting in rel...
- 5Anatomy of a Polymerase - How Function and Structure are ...