Better Than Betaine: PCR Additives That Actually Work
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Ifyou’relikemanyresearchers,problemswithPCRamplifyinghighGCDNAtemplateswillbeamajorannoyanceforyou. Manystrategiesdevelopedtoovercomethisissue. BetaineisthemostcommonPCRadditiveusedtoenhanceamplificationofGCrichsequencesbecauseofitsabilitytodissolvesecondarystructurethatblocksp
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- 1Betaine Enhancer Solution
The performance of Betaine Enhancer Solution is superior compared to ... 5 M Betaine in solution ...
- 2Polymerase Chain Reaction: Basic Protocol Plus ... - NCBI - NIH
- 3Which is better for GC rich amplification: Betaine or DMSO?
5% betain and DMSO from 1% to 5% based on the amount of GC rich gives better results. Try using g...
- 4DMSO and Betaine Greatly Improve Amplification of GC-Rich ...
We further report that DMSO and betaine are highly compatible with all ... of gene synthesis and ...
- 5Better Than Betaine: PCR Additives That Actually Work
Betaine is the most common PCR additive used to enhance amplification of GC rich sequences becaus...
