Blunt-end ligation ratioT4 Ligase NEBCohesive endBlunt end ligation efficiencyLigation protocolSticky endBlunt end restriction enzymesT4 DNA Ligase PromegaA Beginner's Guide to How Blunt-End Cloning Works
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Now let's talk about how blunt-end cloning works. Basically, the blunt-ended insert and linearized vector are mixed with DNA ligase, ... Skiptocontent
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- 1Ligation Protocol with T4 DNA Ligase (M0202) | NEB
- 2DNA Ligation Protocol - Sigma-Aldrich
This kit contains T4 DNA ligase, the enzyme of choice for virtually all cloning purposes because ...
- 3A Beginner's Guide to How Blunt-End Cloning Works
Now let's talk about how blunt-end cloning works. Basically, the blunt-ended insert and linearize...
- 4T4 DNA Ligase Protocol - Promega Corporation
Blunt-end ligations generally are efficient at temperatures between 15–20°C for 4–18 hours, while...
- 5DNA Insert Ligation (sticky-end and blunt-end) into Vector DNA
T4 DNA Ligase. 5 U. Water, nuclease-free to 20 μL. Total volume. 20 μL. This protocol is for the ...
