Polymerase chain reaction testPolymer chain reactionPCR extensionA PCR cycle consist ofPCR templateWhy DNA melting is needed in PCR how this technique was widely applied in clinical diagnostic methodPCR cycle calculationPCR initializationPCR analysis

Amplification efficiency of thermostable DNA polymerases

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DNA polymerase efficiencies were determined under identical conditions using five different amplicon templates that varied in length or percentage GC content. Pfu- and Taq-based formulations showed similar efficiencies when amplifying shorter targets (<900 bp) with 45 to 56%

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