How we can calculate the exact pure PCR fragments after ...
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QuestionAsked1stSep,2015HowwecancalculatetheexactpurePCRfragmentsaftercertaincycles?WhenuseprimerstoperformPCRthentheygenerallydon'tbindattheedgesofbothstrands.WenormallycalculateDNAfragmentofPCRusing2n formulawhereniscyclenumber.Buttoavoidlargeoldfragmenthowwecancalculate?Geth
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Electrophoreseall the samples on a large agarose gel for easy comparison. 3. Calculate the averag...
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The mechanism of PCR copies each DNA fragment with every cycle leading to exponential ... If you ...
- 5How we can calculate the exact pure PCR fragments after ...
We normally calculate DNA fragment of PCR using 2n formula where n is cycle number. But to avoid ...
