PCR Protocol for Taq DNA Polymerase with Standard Taq - NEB
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Mg++ and additives: Mg++ concentration of 1.5–2.0 mM is optimal for most PCR products generated with Taq DNA Polymerase. The Mg-free buffer formulation ... Acceptcookies Weusecookiestounderstandhowyouuseoursiteandtoimprovetheoveralluserexperience.Thisincludespersonalizingcon
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1.DNA 模板：雙股螺旋DNA 經高溫解離成單股DNA，作為PCR 反應的模版。 · 2.Primer 引子：功能就像衛星導航一樣，能找到目標基因片段的頭、尾兩端。
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Magnesium ion (Mg2+) ... Magnesium ion (Mg2+) functions as a cofactor for activity of DNA polymer...
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Excessive magnesium concentrations also stabilize double stranded DNA and prevent complete denatu...
- 4PCR Protocol for Taq DNA Polymerase with Standard Taq - NEB
Mg++ and additives: Mg++ concentration of 1.5–2.0 mM is optimal for most PCR products generated w...
- 5Why does higher magnesium levels lower the PCR band's ...
Higher Mg will make the Taq polymerase more processive and this will increase the yield of the PC...
