Tm 計算機Neb melting temperatureNeb tm primer calculatorTm 溫度 計算Tm Calculator pfuMelting temperature PCRThermo fisher annealing calculatorPhusion dna polymerase Tm CalculatorPCR CalculatorOptimizing Tm and Annealing | Thermo Fisher Scientific - TW
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For primers ≤20nt, use the lower Tm given by the calculator for annealing. For primers >20 nt, use an annealing temperature 3°C higher than the lower Tm given ...
延伸文章資訊
- 1Optimization of the annealing temperature for DNA ... - NCBI
In this article we experimentally determine the optimal annealing temperature (TaOPT) values for ...
- 2FAQs - How do I calculate the correct Annealing Temperature?
The Annealing Temperature to use in a PCR program is based on the value of theoretical Tm (Tm, me...
- 3NEB Tm Calculator
Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR pro...
- 4Tm Calculator | Thermo Fisher Scientific - TW
Application calculates the Tm of primers and estimates an appropriate annealing temperature when ...
- 5What is the relationship between the Tm of primers and ...
Usually annealing temperature is 55-60˚C, but if we lower the temperature i.e. 45-55˚C it promote...
