Colony PCR Exercises | Pathways over time

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Exercise1–PhysicalpropertiesofPCRprimers ThetablebelowliststheprimerpairsusedbytheSGDPtoanalyzethemet deletionmutants.Indesigningtheprimers,researchersaimedforprimerpairsthathadsimilarphysicalproperties,i.e.lengthandTm,andwouldgeneratePCRproductsthatwereseveralhundredbasepair



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