Blunt-end ligation ratioT4 Ligase NEBCohesive endBlunt end ligation efficiencyLigation protocolSticky endBlunt end restriction enzymesT4 DNA Ligase PromegaA simple method for cloning blunt ended DNA fragments
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due to the inefficient ligation of blunt ends by T4 DNA ligase. (1). We have developed a simple procedure for the efficient cloning of blunt ended DNA ... SkiptoMainContent Advertisement SearchMenu
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- 1A simple method for cloning blunt ended DNA fragments
due to the inefficient ligation of blunt ends by T4 DNA ligase. (1). We have developed a simple p...
- 2T4 DNA Ligase Protocol - Promega Corporation
Blunt-end ligations generally are efficient at temperatures between 15–20°C for 4–18 hours, while...
- 3T4 DNA Ligase Protocol - Promega Corporation
- 4How can I perform a blunt-end ligation with a large vector and ...
My protocol thus far has been as follows: 1. Restriction digest of backbone vector (7,600 bp) wit...
- 510 ways to improve blunt-end ligations - Bitesize Bio
