Blunt-end ligation ratioT4 Ligase NEBCohesive endBlunt end ligation efficiencyLigation protocolSticky endBlunt end restriction enzymesT4 DNA Ligase PromegaProtocol - How to Ligate Plasmid DNA - Addgene
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Protocol: Standard Insert + Vector DNA Ligation ... the length of the DNA to get a proper ratio of 3 available insert ends for every available vector end. Skiptomaincontent Protocols DNALigation
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- 1How can I perform a blunt-end ligation with a large vector and ...
My protocol thus far has been as follows: 1. Restriction digest of backbone vector (7,600 bp) wit...
- 2Protocol - How to Ligate Plasmid DNA - Addgene
Protocol: Standard Insert + Vector DNA Ligation ... the length of the DNA to get a proper ratio o...
- 3Blunt-end cloning method protocol
a challenge due to the inefficient ligation of blunt ends by T4 DNA ligase. This protocol demonst...
- 4DNA Ligation Protocol - Sigma-Aldrich
This kit contains T4 DNA ligase, the enzyme of choice for virtually all cloning purposes because ...
- 5T4 DNA Ligase | NEB
