Application of Polynucleotide Kinase

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Polynucleotide kinase as a potential target for enhancing cytotoxicity ...Since human polynucleotide kinase (hPNK), the DNA repair enzyme that is the subject of ... The bacteriophage T4 uses its PNK, also a bifunctional kinase and ... tw | twT4 Polynucleotide Kinase | NEBApplication Features · End-labeling DNA or RNA for probes and DNA sequencing (2) · Addition of 5´-phosphates to oligonucleotides to allow subsequent ligation ... twphosphatase activity of mammalian polynucleotide kinase takes ...The dual function mammalian DNA repair enzyme, polynucleotide kinase (PNK), ... enzyme were incubated on ice for 20 min before application to the membrane.Sensitive and label-free T4 polynucleotide kinase/phosphatase ...Furthermore, the application of the proposed strategy for screening T4 PNKP inhibitors also demonstrated satisfactory results. With the advantages mentioned ...T4 Polynucleotide Kinase Technical Bulletin - Promega CorporationA protocol for use with T4 Polynucleotide Kinase to transfer of the γ-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides ... | T4 Polynucleotide Kinase - Promega CorporationWe use cookies and similar technologies to make our website work, ... T4 Polynucleotide Kinase catalyzes the transfer of the γ-phosphate from ATP to the ... | Polynucleotide kinase–phosphatase enables neurogenesis via ...Polynucleotide kinase–phosphatase (PNKP) is a DNA repair factor possessing ... we generated multiple mutant Pnkp alleles to use as a tool to understand how ...The Interaction between Polynucleotide Kinase Phosphatase and ...Parsons J.L.; Dianova I.I.; Allinson S.L.; Dianov G.L.. DNA polymerase β promotes recruitment of DNA ligase III α-XRCC1 to sites of base excision repair.Linker region is required for efficient nuclear localization of ... - PLOS2020年9月24日 · Polynucleotide kinase phosphatase (PNKP) is a DNA repair factor with ... which permits unrestricted use, distribution, and reproduction in ...XRCC1 Stimulates Human Polynucleotide Kinase Activity at ...Here, we report that XRCC1 interacts with human polynucleotide kinase in addition to its established interactions with DNA polymerase-β and DNA ligase III.