Polynucleotide kinase PDF

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[PDF] Datasheet for T4 Polynucleotide Kinase - NEBTo simplify this process, T4 Polynucleotide Kinase is optimized for use in T4 DNA Ligase reaction buffer (which contains the appropriate amount of ATP). We ... twT4 Polynucleotide Kinase - Promega CorporationT4 PNK catalyzes the transfer of the gamma-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a 5´-hydroxyl group.Polynucleotide kinase as a potential target for enhancing cytotoxicity ...Strand breaks that are caused by many agents including ionizing radiation, topoisomerase I inhibitors, and DNA repair glycosylases such as NEIL1 and NEIL2, ...Phosphatase Component of T4 Polynucleotide Kinase ... - NCBIT4 polynucleotide kinase/phosphatase (Pnkp) is the prototype of a family of repair enzymes that heal broken termini in RNA or DNA by converting 3′PO4/5′OH ends ...Mechanism of DNA substrate recognition by the mammalian DNA ...2009年8月11日 · Abstract. Mammalian polynucleotide kinase (mPNK) is a critical DNA repair enzyme whose 5′-kinase and 3′-phoshatase activities function with ...phosphatase activity of mammalian polynucleotide kinase takes ...Abstract. The dual function mammalian DNA repair enzyme, polynucleotide kinase (PNK), facilitates strand break repair through catalysis of 5′-hydroxyl ...Polynucleotide kinase–phosphatase enables neurogenesis via ...Polynucleotide kinase-phosphatase (PNKP), a repair factor that modifies DNA breaks prior to ligation, is essential for normal brain development by preventing ...Dual Modes of Interaction between XRCC4 and Polynucleotide ...sociated domain of the end-processing enzyme polynucleotide kinase/phosphatase (PNKP). Here we reveal that unphosphory- lated XRCC4 also interacts with PNKP ...Sensitive and label-free T4 polynucleotide kinase/phosphatase ...T4 polynucleotide kinase/phosphatase (PNKP) has the capacity to ... The fluorescence measurements were carried out on an FL-4600 spectrometer (Hitachi, ...[PDF] T4 Polynucleotide Kinase, Cloned Manual - LucigenT4 Polynucleotide Kinase, Cloned (T4 PNK) catalyses the transfer of the γ-phosphate of ATP to the 5′ terminus of single- and double-stranded DNA or RNA ... tw