DNA ligase
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DNA Ligase Selection Chart | NEBDNA Ligase Selection Chart. While more than one ligase may work for your application, the following selection chart presents our recommendations for optimal ...DNA Ligases: Progress and Prospects - NCBI2009年3月27日 · Crystal structures of DNA ligases bound to nucleotide and nucleic acid substrates have illuminated how ligase reaction chemistry is catalyzed, ...T4 DNA ligase structure reveals a prototypical ATP-dependent ...2018年8月29日 · DNA ligases play essential roles in DNA replication and repair. Bacteriophage T4 DNA ligase is the first ATP-dependent ligase enzyme to be ...Engineered DNA ligases with improved activities in vitroThe DNA ligase from bacteriophage T4 is one of the most widely used enzymes in ... ligase-FL-cTF and ligase-HL-cTF are described in the Supplementary data.Differential recruitment of DNA Ligase I and III to DNA repair sitesAbstract. DNA ligation is an essential step in DNA replication, repair and recombination. Mammalian cells contain three DNA Ligases that are not ...The Inhibitory Effect of Non-Substrate and Substrate DNA on ... - PLOS2016年3月8日 · DNA ligases are essential both to in vivo replication, repair and recombination processes, and in vitro molecular biology protocols.Two-tiered enforcement of high-fidelity DNA ligation - Nature2019年11月28日 · DNA ligases catalyze the joining of DNA strands to complete DNA replication, recombination and repair transactions. To protect the integrity ...Full article: DNA ligase III - Taylor & Francis OnlineDNA ligases are crucial for most DNA transactions, including DNA replication, repair, and recombination. Recently, DNA ligase III (Lig3) has been ...Elevated Expression of DNA Ligase I in Human CancersIn DNA ligation reactions mediated by mammalian DNA ligases, ATP is used as a cofactor to form a covalent enzyme-adenylate complex, and in subsequent steps, the ...T4 DNA Ligase - Sigma-AldrichT4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA.
延伸文章資訊
- 1DNA連接酶- 维基百科,自由的百科全书
DNA連接酶(EC 6.5.1.1),也称DNA黏合酶,在分子生物學中扮演一個既特殊又關鍵的角色,那就是把兩條DNA黏合成一條。無論是雙股或是單股DNA的黏合,DNA連接酶都可以藉 ...
- 2DNA连接反应
Overview. 分子生物学中,连接是指通过形成磷酸二酯键将两个DNA片段连起来。连接反应是由一种叫连接酶的酶催化进行的。在细胞内,连接酶用于修复在DNA复制过程中的单链 ...
- 3T4 DNA Ligase(T4 DNA连接酶)(D7008) - 碧云天
T4 DNA Ligase即T4 DNA连接酶,可以催化粘端或平端双链DNA或RNA的5'-P末端和3'-OH末端之间以磷酸二酯键结合,该催化反应需ATP作为辅助因子。同时T4 DNA 连接酶可...
- 4Taq DNA连接酶 - 翊圣生物
Taq DNA Ligase是一种耐高温连接酶,它能催化与同一互补靶DNA链杂交的两条相邻寡核苷酸链的5´-磷酸和3´-羟基之间形成磷酸二酯键。该催化反应只有当两条寡核苷酸链与 ...
- 5DNA连接酶_百度百科
DNA连接酶(DNA Ligase)也称DNA黏合酶,在分子生物学中扮演一个既特殊又关键的角色,那就是连接DNA链一个碱基3'-OH末端和它相邻碱基的5'-P末端,使二者生成磷酸二酯键, ...