T4 DNA Ligase
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T4 DNA Ligase | NEBT4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. twT4 DNA ligase structure reveals a prototypical ATP-dependent ...2018年8月29日 · DNA ligases play essential roles in DNA replication and repair. Bacteriophage T4 DNA ligase is the first ATP-dependent ligase enzyme to be ...Comparative analysis of the end-joining activity of several DNA ligases2017年12月28日 · This analysis revealed that T4 DNA ligase, the most common enzyme utilized for in vitro ligation, had its greatest activity on blunt- and ...Engineered DNA ligases with improved activities in vitroThe DNA ligase from bacteriophage T4 is one of the most widely used enzymes in ... ligase-FL-cTF and ligase-HL-cTF are described in the Supplementary data.Standard Free Energy for the Hydrolysis of Adenylylated T4 DNA ...Equilibrium constants for the adenylylation of T4 DNA ligase have been measured at 10 pH values. The values, when plotted against pH, fit a titration curve ...Template‐independent ligation of single‐stranded DNA by T4 DNA ...2005年11月16日 · T4 DNA ligase is one of the workhorses of molecular biology and used in various ... Email; Facebook; Twitter; Linked In; Reddit; Wechat ...The phage T4 DNA ligase in vivo improves the survival-coupled ...2019年6月13日 · The T4 DNA ligase is one of the most widely utilized enzymes in modern molecular biology. Its contribution to repair chromosomal DNA damages ...adenylation by T4 DNA ligase to facilitate practical applications2006年4月17日 · In our previous report (Silverman 2004), the protein enzyme T4 RNA ligase was used along with a complementary DNA oligonucleotide (Fig. 1B) to ...T4 DNA Ligase (5 U/µL) - Thermo Fisher ScientificThermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or ... twT4 DNA Ligase - Sigma-AldrichT4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. |
延伸文章資訊
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DNA連接酶(EC 6.5.1.1),也称DNA黏合酶,在分子生物學中扮演一個既特殊又關鍵的角色,那就是把兩條DNA黏合成一條。無論是雙股或是單股DNA的黏合,DNA連接酶都可以藉 ...
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DNA連接酶(DNA ligase) ... 在分子生物學中扮演一個既特殊又關鍵的角色,那就是把兩條DNA片段(DNA fragments)黏合成一條。
- 4T4 DNA Ligase(T4 DNA连接酶)(D7008) - 碧云天
T4 DNA Ligase即T4 DNA连接酶,可以催化粘端或平端双链DNA或RNA的5'-P末端和3'-OH末端之间以磷酸二酯键结合,该催化反应需ATP作为辅助因子。同时T4 DNA 连接酶可...
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DNA損傷因素可以分為DNA內生性損傷與DNA外生性損傷,內生性損傷(如核苷酸鹼基 ... 單鏈斷裂重接, 一部分可由DNA連接酶(ligase)參與而完全修復;此酶在各類生物細胞中都 ...