T4 DNA Ligase Thermo
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T4 DNA Ligase (5 U/µL) - Thermo Fisher ScientificThermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or ... twT4 DNA Ligase (1 U/µL) - Thermo Fisher ScientificT4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3 hydroxyl and 5 phosphate termini. twOPTIZYME™ T4 DNA Ligase, Fisher BioReagentsMilliporeSigma ™ Novagen ™ KOD Hot Start DNA. $308.00 / Each. Thermo Scientific™ T4 DNA Ligase, LC (1 U/µL). Catalog No. FEREL0016.Comparative analysis of the end-joining activity of several DNA ligases2017年12月28日 · This analysis revealed that T4 DNA ligase, the most common enzyme utilized for in vitro ligation, had its greatest activity on blunt- and ...T4 DNA ligase structure reveals a prototypical ATP-dependent ...2018年8月29日 · Bacteriophage T4 DNA ligase is the first ATP-dependent ligase ... the same buffer in a 2 kDa cutoff Slide-A-lyzer cassette (Thermo Fisher).DNA unwinding and inhibition of T4 DNA ligase by anthracyclines.The ability to alter DNA tertiary structure of ten anthracycline derivatives whose antitumor potency is known was studied by an assay that makes use of nicked ...圖片全部顯示Archaeal RNA ligase from thermoccocus kodakarensis for template ...Chlorella virus DNA ligase, T4 DNA ligase, and T4 RNA ligase 2 are all monomeric ... When a pre-treatment step with thermo-labile proteases is performed, ...single-molecule sequencing assay for the comprehensive profiling ...2018年5月8日 · This method has been used to profile the ligation of all three-base 5′-overhangs by T4 DNA ligase under typical ligation conditions in a single ...A high-efficiency method for site-directed mutagenesis of large ...2021年5月17日 · DNA ligation was performed in a 20 μL reaction mixture containing 5 μL phosphorylated samples and 5 U T4 DNA ligase (Thermo Scientific).
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T4 DNA Ligase即T4 DNA连接酶,可以催化粘端或平端双链DNA或RNA的5'-P末端和3'-OH末端之间以磷酸二酯键结合,该催化反应需ATP作为辅助因子。同时T4 DNA 连接酶可...
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DNA連接酶(DNA ligase) ... 在分子生物學中扮演一個既特殊又關鍵的角色,那就是把兩條DNA片段(DNA fragments)黏合成一條。