How Much Template Will I Add to My PCR Reaction?
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Even though in theory, one molecule of the template would be sufficient, considerably larger amounts of DNA are typically used for a classic ... HomeSampleHandlingAmplification
延伸文章資訊
- 1Effect of PCR template concentration on the ... - PubMed
Purified DNA was used as template either undiluted or diluted 1:10 prior to polymerase chain reac...
- 2Working with PCR - Roche Life Science
The necessary amount of template varies from reaction to reaction. As a guideline, use 100 - 750 ...
- 3Cloning - PCR Strategy - Optimising PCR - EMBL Heidelberg
- 4Guidelines for PCR Optimization with Taq DNA Polymerase
Magnesium Concentration · 1.5-2.0 mM is optimal for Taq DNA Polymerase · Optimal concentration de...
- 5Effects of polymerase, template dilution and cycle number on ...
The primer and amplicon length have been found to affect PCR based ... the template dilution fold...
