Effects of polymerase, template dilution and cycle number on ...
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The primer and amplicon length have been found to affect PCR based ... the template dilution fold rather than the absolute concentration, ... Skiptomaincontent Advertisement SearchallBMCarticles Search
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- 1Guidelines for PCR Optimization with Taq DNA Polymerase
Magnesium Concentration · 1.5-2.0 mM is optimal for Taq DNA Polymerase · Optimal concentration de...
- 2Polymerase Chain Reaction: Basic Protocol Plus ... - NCBI
The usual dNTP concentration is 50 μM of EACH of the four dNTPs. However, PCR can tolerate concen...
- 3Cloning - PCR Strategy - Optimising PCR - EMBL Heidelberg
- 4Effects of polymerase, template dilution and cycle number on ...
The primer and amplicon length have been found to affect PCR based ... the template dilution fold...
- 5How Much Template Will I Add to My PCR Reaction?
Even though in theory, one molecule of the template would be sufficient, considerably larger amou...
