Melting Temperature (Tm) Calculation
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Melting Temperature (Tm) Calculation. Primer (6-50 bases):. LENGTH 20 C+G% 25 Molecular weight: 6272.715. Basic Tm Degenerated nucleotides are allowed. MeltingTemperature(Tm)CalculationPrimer(6-50bases):LENGTH20 C+G%25 Molecularweight:6272.715 BasicTmDegeneratednucleotidesarea
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Tm=ΔHkcal∘C×MolΔS + R In ([primer] / 2)−273.15∘C. ΔH is the enthalpy of base stacking interaction...
