Tm Calculator | Oligo melting temperature - Promega ...
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Tm=ΔHkcal∘C×MolΔS + R In ([primer] / 2)−273.15∘C. ΔH is the enthalpy of base stacking interactions adjusted for helix initiation factors (3,4). Ourwebsitedoesnotfullysupportyourbrowser. We'vedetectedthatyouareusinganolderversionof
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- 1引子合成Primer Synthesis - 百力生物科技股份有限公司
Tm 計算公式. 百力計算Tm 值的公式如下： If Size ≦14 Tm = (NA + NT) ×2 + (NC + NG) × 4 If Size >14 Tm = 64.9 + 41 ...
- 2Tm Calculator | Thermo Fisher Scientific - TW
Application calculates the Tm of primers and estimates an appropriate annealing temperature when ...
- 3OligoCalc: Oligonucleotide Properties Calculator
None of the following melting temperature (Tm) calculations provide ... the OligoCalc DNA MW calc...
- 4Melting Temperature (Tm) Calculation
Melting Temperature (Tm) Calculation. Primer (6-50 bases):. LENGTH 20 C+G% 25 Molecular weight: 6...
- 5TM Calculator - Horizon Discovery
If the PCR primer contains desired mismatches, e.g. for creating a mutation or a restriction site...
