PCR Cycling Parameters—Six Key Considerations for ...

Overlap extension PCR · Annealing parts of primer: Solid arrow in scheme; Should bind to template...

A typical PCR cycle includes an extension step at 72°C after denaturation of double-stranded DNA ...

DNA denaturation considerations; Primer annealing optimization. Primer extension considerations; ...

Final Extension for 5 minutes at 72°C: A final extension to fill-in any protruding ends of the ne...

Extension is achieved by using the loosened nucleotides of each base to grow the complementary DN...